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Support for GHK-Cus systemic effects is based principally on animal and in vitro studies, but the findings give reason to be optimistic about its potential as an injectable treatment

Adenosine triphosphate (ATP) detection Total cellular ATP levels were determined using a bioluminescent ATP assay kit (Beyotime)

Cellular uptake, intracellular trafficking, and cytotoxicity of nanomaterials

For researchers designing combination protocols, this approach makes mechanistic sense but requires dose-optimization work to avoid redundant effects or unexpected interactions

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