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The RNAprotein complex was captured by streptavidin-couped Dynabeads (Thermo, 65606D) (room temperature, 30 min), washed with ice-cold 1 TENT buffer, mixed with 1 Laemmli sample buffer and heated for 10 min at 95 C for immunoblot analysis using anti-YBX1 antibody (ABclonal, A3534)

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Unlike oral supplements, which can be poorly absorbed, intravenous delivery bypasses the digestive system, ensuring that the body can quickly utilize these vitamins for various metabolic functions