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Associations between immune biomarkers and symptoms of anxiety, depression, and insomnia in pediatric inflammatory bowel disease: a preliminary longitudinal analysis

The results did not correspond with the a priori hypothesis of immunosuppression in MDD

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A 100 L volume of supernatant was transferred to a microtube and mixed with 100 L of 2, 4 nitrophenylhydrazine (DNPH) prepared as a 5 mM solution in 2 M hydrochloric acid

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