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A COA is a document issued by an independent testing laboratory that verifies a compound's identity, purity, concentration, and molecular weight

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For elution of the target protein, the interaction to the ligand is resolved by changing the buffer conditions, for example, the pH value, or by adding a specific competitor that supplants the target protein from the ligand resulting in dissociation of the target, while the ligand remains immobilized on the stationary phase

An alternative possibility involving S-2,4-dinitrophenylation of thiols or other cellular nucleophiles was excluded based on the lack of an equal effect of CDNB, which could produce a similar arylation without release of NO