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The injection volume was 0.3 ml/mouse

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Factors Indicating Against Dose Escalation Maintain current dose when experiencing significant side effects at present level, achieving satisfactory subjective improvements (better sleep, energy, recovery), IGF-1 increased adequately (50+ ng/mL) for optimization goals, or financial constraints make higher dosing unsustainable

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To this end, many drugs have been repurposed, including tocilizumab, sarilumab, heparin, chloroquine, ivermectin, sarilumab, chromones (Sestili and Stocchi, 2020)