LCMS/MS analysis was performed using an Advance nanoLC (BrukerMichrom, Auburn, CA, USA) and LTQ linear ion trap mass spectrometer (Thermo Fisher Scientific) equipped with a NANOHPLC capillary column C18 (0.075 mm ID 150 mm length, 3 m particle size, Nikkyo Technos, Tokyo, Japan) using a linear gradient (25 min, 535% CH 3 CN /0.1% formic acid) at a flow rate of 300 nL/min
2C), or the ratio of free GSH to GSSG (Fig
References Aimone J.B., Wiles J., Gage F.H
The model was defined by five differential equations: T 1 , T 2 , and T 3 are the amount of drug in the transit compartments, C and P are the amount of drug in the central and peripheral compartments, k a and k el are the first-order absorption and elimination rate constants, k 12 and k 21 are the first-order transfer rate constants between the central and peripheral compartments, V/F is the volume of distribution, F is the bioavailability, and Cp is the drug concentration in the central compartment
Oral glutathione supplementation is largely ineffective because glutathione is broken down in the gut before absorption
