Cells were washed with PBS, detached with trypsin and centrifuged (5 min, 400 g), resuspended and covered with 5 M working solution of MitoSOX reagent in HBSS and incubated at 37 C for 10 min in FACS tubes
[8] Compared with saline, delayed T4 did not shrink the lesion volume a telling detail, because it means the benefit was not simply less initial damage yet it significantly reduced hippocampal cell loss, enhanced angiogenesis and neurogenesis in the injured cortex and hippocampus, increased oligodendrogenesis, and improved sensorimotor recovery and spatial learning
Rapid improvement in glucose control has been associated with a temporary worsening of diabetic retinopathy
Researchers on these higher doses typically use 20 mg/mL or higher concentrations to keep volumes manageable
Brunner AL, Johnson DS, Kim SW, Valouev A, Reddy TE, Neff NF et al