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In particular, key amino acids or intermediate metabolites involved in de novo or salvage synthesis of glutathione (GSH), particularly cysteine (Cys), - glutamylcystine (-GC), glutamine (Gln), glutamate (Glu), and GSH itself, were significantly decreased in LDHB KD A549 cells compared with those in control A549 cells expressing control siRNA (siNT) ( Figure 1c, d )

2.4 Amino acid metabolism Amino acids are the building blocks of proteins and serve as raw materials for intracellular protein synthesis

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