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Each member of the treatment groups received an icy injection of scopolamine hydrobromide (70 nmol in 2 l aCSF over a duration of 20 s) 20 min prior to testing followed by Dihexa (300 pmol in 2 l aCSF), Hinge (300 pmol in 2 pl aCSF), or Hinge+Dihexa (300 pmol in 4 l aCSF) 5 min prior to testing

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Dead animals are thoroughly documented or collected for comprehensive internal examinations

Getting the dose exactly right at this phase matters because it establishes your baseline response before deciding whether to increase further