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As our in vivo scRNA-seq data did not wholly capture the heterogeneity reflective of this progression, we studied the process by culturing basal tracheal epithelial cells at airliquid interface (ALI) and harvesting replicate cultures at 20 timepoints across mucociliary differentiation for scRNA-seq analysis (Fig

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Third, although our study showed significant changes in ion channel function and the expression of inflammation and airway cell markers in response to PG/VG aerosols after seven days, longer-term exposures may be necessary to mimic the effects of chronic e-cig use in humans

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