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After 2 h of treatment, 10 M of the fluorescence probe 2,7-dichlorofluorescein diacetate (DCFH-DA, D6883, Sigma-Aldrich, MO, USA) was added to the cells

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Moreover, summary statistics such as average daily observations and the ratio of days with observations relative to the total tracking period were calculated to quantify patient engagement

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When dokha is packed too tightly, it restricts airflow, making it difficult to draw smoke and often resulting in an uneven burn