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Also oral administration, although with conflicting results, resulted in increased serum GSH levels with reduced oxidative stress and beneficial effects in several diseases (27, 28)

2 Biosynthesis of melanin and its regulation 2.1 Melanin synthesis pathway The specific process of melanin synthesis begins with the conversion of tyrosine into L-Dopa catalysed by tyrosinase, the central glycoprotease in the melanosome region and the only rate-limiting enzyme in this process (Kim et al., 2023)

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The glutathione content of the hepatocytes was determined by Ellman`s method using spectrophotometer

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