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Another recent study showed increased calcium accumulation in renal tubular epithelial cells in response to CHAC1 overexpression, which was ameliorated by CHAC1 knockdown ( CHAC1 upregulation was induced by cysteine and methionine deprivation, treatment with erastin and exposure to tunicamycin

P values indicated, calculated by two-way ANOVA with Tukeys ( a ) or dks multiple comparisons test ( b,c ), and one-way ANOVA with Dunnetts multiple comparisons test ( d )

Apoptotic cells were estimated by the percentage of cells that stained positive for Annexin V-FITC

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