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Bait proteins used for pull-down assays can be either ready-to-use purified proteins by linking an affinity tag or producing recombinant fusion-tagged proteins

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Hence, the following affinity score, called Yassers number one (YN1), was employed:where is ligand efficiency, is molecular weight, is the log base 10 of IC50, is the maximum value inside the cluster, is the minimum value inside the cluster, and is the number of molecules of the cluster

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