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It turned out that most things other than water, protein, and some salts and sugar were performance impairers

Clin Cancer Res 23(2):587599 Karakhanova S et al (2015) Characterization of myeloid leukocytes and soluble mediators in pancreatic cancer: importance of myeloid-derived suppressor cells

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Histochemical and immunohistochemical staining Tissue cryosections were fixed for 15 min, incubated with SA--gal staining at 37 C overnight in the dark according to the manufacturers instructions (Solarbio, Beijing, China)

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