Rec J Exonuclease, 250 U @ 10 U/uL GFRNASC Additional substitutions of 2´-dNTPs (or

Additional substitutions of 2´-dNTPs (or 2´-modified-dNTPs) for canonical rNTPs will further reduce yield of the transcript produced

surfactants

human β-globin

Expressing Plasmidsc

and Vogelstein

Rec J Exonuclease, 250 U @ 10 U/uL GFRNASC Additional substitutions of 2´-dNTPs (orMagnesium dependent digestion of single stranded DNA in a 5 to 3 direction Specific: Digests ssDNA in the 5' to 3 direction Heat Inactivated: Heat at 65C for 20 minutes to kill enzyme activity Flexible: Use to remove primers from completed PCR or to degrade single stranded linear DNA in dsDNA and plasmid preps Rec J Exonuclease, derived from E. coli, catalyzes degradation of ssDNA in a 5 to 3 direction. Its activity is dependent on Mg2+. Rec J