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Of all the 145 male patients, 60 were smokers and 85 were nonsmokers

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Medium was then exchanged for EV-depleted complete culture medium, and cells were incubated for 3 days, after which medium was collected from each flask and stored at 80 C

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In the mid-1990s, the Federal Ministry of Health recommended several tobacco control measures, but failed to provide funding for their enactment