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After 5 min of incubation at room temperature in plain RPMI-1640 medium, 100 nM Calcein-AM (Sigma-Aldrich, Germany) was added, and cells were incubated for 10 min at 37 C

[DOI] [PMC free article] [PubMed] [Google Scholar] 212.Mule S., Ferrari S., Rosso G., Brovero A., Botta M., Congiusta A., Galla R., Molinari C., Uberti F