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To identify drug-exposure conditions with potentially high tumor specificity, we first assessed the response of tumoral and BBB cells to four chemotherapeutic drug compounds (cisplatin, doxorubicin, homoharringtonine (HHT), and docetaxel) in a well-plate assay, by exposing DMG and BBB spheroids to 10 different drug concentrations in ULA plates

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SIRT3, a class III HDAC associated with longevity, deacetylates Forkhead box protein O3a (FOXO3a) and promotes transcription of antioxidant enzymes (e.g., manganese superoxide dismutase and catalase), thereby constraining mitochondrial ROS and preserving redox homeostasis [71, 72]

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Reaction Components and Energy Systems In this section, we describe the components most frequently found in cell-free reaction mixtures to activate metabolism, transcription, and translation (Figure 3)