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Similarly, in experiments with T4 ip2 ip3 and the Tn5 library constructed with ECOR17 (which resists infection by T4 ip2 ip3 using both O-antigen and GmrSD ECOR17 ), MOI was titrated in five parallel infection screens (with varying amounts of phage lysate added: 200 l, 50 l, 12.5 l, 2.5 l, 1 l)

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Transduced DG-75 cells (2 10 6 ) in 0.1 ml of phosphate buffer saline (PBS) were injected subcutaneously in the dorsal flanks of 8-to 10-week-old female NOD.CB17-Prkdcscid/J mice (Charles River Laboratories, Wilmington, MA, USA)