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While Retatrutide appears to preserve muscle better than single-receptor agonists, some degree of lean mass loss is still expected during significant caloric deficit

Antibiotic medium (MEM, 2% PS, 12.5 g/mL amphotericin B, 0.5 mg/mL gentamicin sulfate) was added and the cells were cultured in an incubator (28C + 5% CO2) for 2 hours, with a medium change every 30 minutes

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Step 2: Clean vial stoppers with alcohol swabs

AE-F: Data curation, Formal Analysis, Investigation, Methodology, Software, Visualization, Writingoriginal draft, Resources, SFI: Data curation, Formal Analysis, Funding acquisition, Resources, Software, Validation, Writingoriginal draft, Writingreview and editing