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However, the promotion or inhibition of HO-1 for ferroptosis has obtained contradictory results, so the relationship between them needs to be further explored [41, 42]

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After 24 h, viability was assessed using the AquaBluer assay as previously described ( Determination of GSH Levels HT-1080 cells were cultured in DMEM containing 10% FBS, 1% supplemented non-essential amino acids and 1% Pen-Strep mixture (all from Gibco), and maintained in a humidified atmosphere with 5% CO 2 at 37C

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Redox Indicator: The GSH/GSSG ratio is widely used as a readout of cellular oxidative stress and redox state