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Blood Thinners and Anticoagulants Anticoagulants, particularly warfarin and heparin, frequently cause hair loss through disruption of the anagen growth phase

Hellinger R, Sigurdsson A, Wu W, Romanova EV, Li L, Sweedler JV

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Once the lysate had been loaded, the column was washed with 20 column volumes (CV) wash buffer, and the His-tagged TRFH domain was eluted using a linear gradient from 0 to 100% elution buffer (50 mM Tris-HCl pH 8.0, 500 mM NaCl, 1 mM TCEP, 300 mM imidazole) over 25 CV