After washing three times with TBST, membranes were incubated with HRP-conjugated secondary antibody and visualized with enhanced chemiluminescence
To investigate the effect of gene knockout on l-cysteine and GSH degradation, the engineered strains were cultured in the flasks as described above and the cells were collected by centrifugation (6000 rpm, 4 C for 5 min) after two washes with 50 mM phosphate buffer (pH7.0)
& Osswald, W
These pathways are crucial for neutrophil survival and function since blocking them can make individuals more susceptible to ferroptosis (66)
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