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In brief, HUVECs were lysed in Co-IP buffer containing 20 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.5 mM EDTA, 0.5% NP-40, 0.5 mM PMSF, and 0.5% protease inhibitor cocktail on ice for 20 min

However, the frequency of injections may vary depending on your individual needs

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In fact, selenite, the most common form of dietary inorganic selenium, is rapidly transformed to selenide and enters this metabolic pathway directly through that selenide door. One can understand that it is then rapidly incorporated in the seleno-cysteinyl moiety and activates Se-GSH-Px