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Immunoblotting analyses with the indicated antibody for MES20, MES28 and GSC2907 GSCs (n = 3), respectively

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After blocking the cellular antigen with 3% bovine serum albumin at room temperature for 1 hr in the presence of 0.1% Triton X-100, cells were incubated overnight at 4C with respective primary antibodies: mouse anti-GLUT1 (1250 dilution) and rabbit anti-GFAP (1200 dilution) for astrocytes

Minor side effects like nausea or headache are possible but not the norm, and serious complications are rare

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