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Purification of CTR/AMYR-DNGs complex in complex with peptide ligands High-titre baculovirus was generated by transfecting recombinant bacmid (CTR, RAMP and DNG s ) or plasmid (G12) into Sf9 cells using the Bac-to-bac baculovirus system (Invitrogen)

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In the second and fourth cycles, the animals were administered 6, 30, and 150 g/kg BPC157 saline solutions via single IM injections

Even a single amino acid substitution can dramatically alter the peptide's properties and render it ineffective