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3.3 Determination of strength of interaction of CIPp-18 with BSA To determine the strength of interaction of CIPp-18 with BSA, a BenesiHildebrand (BH) plot of changes in Soret band absorption of CIPp-18 vs different concentration of BSA was constructed and the albumin-binding constant of CIPp-18 was computed from the BH plot as reported in several studies [44,45,46,47,48] using the following equation: where K a is the binding constant, A 0 , A, and A are the absorbances of CIPp-18 in the absence, presence of specified, and infinite concentration of BSA respectively