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Intrinsic factor, a protein that shuttles B12 through the absorption process, becomes saturated after a meal containing an above-average amount of B12 and about 4 to 6 hours are required before it can efficiently absorb another dose (IOM, 1998)

Furthermore, EPR obtains signal from all spins, whether they are part of insoluble or soluble assemblies

Severe or prolonged deficiency can cause nerve damage and other serious health issues if untreated.