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"Because nicotine can alter the size of the blood vessels and the ability for the body to adapt, that can cause increased damage to the ligaments as well as muscles within the spine region," says rehabilitation specialist Anand Navarasala (via Franciscan Health)

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After 2 h, the secondary antibody was removed and the membrane was incubated with SuperSignal West Pico Chemiluminescent substrate for imaging using a ChemiDoc Imaging System (BIO-RAD)