Intensity of behavioral disturbances and seizure presentation assessment After paracetamol application, intensity of progressing behavioral disturbances and seizure presentation was continuously monitored, and assessed and scored (score 0 - normal, score 1 - rearing on hind legs, shaking, 2 - sleepy, tottering walk, 3 - sleepy, tottering walk and repeatedly falling down, 4 - sleepy, tottering walk and repeatedly falling down, jerking of the extremities, 5 - lying down, generalized convulsions) at particular time points depending on the regimen used, as described before
After the control and experimental groups were formed, all cells were incubated with FBS-free DMEM overnight for serum starvation
Here, we are constantly producing a wide range of life-saving drugs for your safe future
Center for Internal Medicine
After 5 d of culture, cells were stained for beta-III tubulin, then counterstained with 0.1 M DAPI (D9542