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The supernate was then passed through 0.22 mm syringe filters (Millipore) and loaded onto a Ni Sepharose High performance column (GE Healthcare, 5mL), washed with five column volumes of 40 mmol/L imidazole, and eluted with 400 mmol/L imidazole

In 2014, a toddler died from accidental liquid nicotine poisoning

The primary objective of Djarum D was to provide a distinct alternative to the King of Kretek, the Dji Sam Soe 234, by offering a refined flavor profile and superior moisture protection