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For the proteomic analysis, 300 L aliquots of urine were centrifuged twice at 10,000 g for 20 min at 4C to separate urinary proteins from small molecules using an Amicon Ultra 0.5 ml Centrifugal Filter (10 kDa, UFC501096, Merck Millipore, Darmstadt, Germany) according to the manufacturers instructions

However, there are open questions how plasma-generated RONS interact on a molecular level in a biological environment, e.g

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