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AmpliSeq of bulk RNA samples For each donor/timepoint, 0.53 10 5 cells were aliquoted from those harvested for scRNA-seq and bulk RNA was extracted with the Quick-RNA Microprep Kit including on-column DNase treatment

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In order to investigate JHM in joints other than those included in the Beighton score, standards are available for adult (i.e., 16 years of age) subjects (Table 8) [147]

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