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They found that BVC not only enhances the immune recognition between CD8 + T cells and FAS-overexpressing tumor cells but also reduces tumor cell immune escape through PD-1/PD-L1 blockade, resulting in significant benefits for the eradication of metastatic tumors [171]

Twelve weeks after the first inoculation, animals were anaesthetized with 5% (vol/vol) isoflurane followed by cervical dislocation