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However, certain populations are at higher risk of deficiency: Adults over 50 (reduced absorption capacity) Individuals following vegan or strict vegetarian diets Those with gastrointestinal conditions such as Crohns disease or celiac disease Individuals taking long-term acid-reducing medications (PPIs, H blockers) When oral supplementation is insufficient or absorption is compromised, vitamin B12 shots deliver the nutrient directly into the bloodstream, bypassing the digestive system entirely

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The mice were initially anesthetized with pentobarbital (50 mg/kg body weight) administered through intraperitoneal injection

Studies in rats and beagle dogs have shown a rapid elimination half-life of less than 30 minutes after intravenous administration