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10 Discussion Cancer is the leading cause of death and immunotherapy has attracted a wide range of attention due to its advantages such as durable response, long-term survival benefit, and lower toxicity and side effects (242, 243), in which immune cells are the target cells for immunotherapy, which as part of the TME are mainly composed of TAMs, DCs, NK cells, TANs, MDSCs and T cells

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The critical point: you cannot visually detect denaturation

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