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Individual results vary based on age, overall health, and consistency

Preparation Before the injections, the treatment area is cleaned, and a topical numbing cream may be applied to minimize discomfort

Song L, Rape M

After withdrawal of a blood sample, the mice were allowed to recover for at least 1 day and the average running wheel activity over the next two days was taken as baseline value

AP/NTS and LPBN sections were incubated at 4 C for 48 h with rabbit anti-Fos antibody (1:500, Cell signalling technology, Switzerland, Cat# 2250, RRID: AB_2247211), and then after PBS washes, incubated with goat anti-rabbit Alexa Fluor CY3 (1:200, LuBio Science GmbH, Switzerland for Jackson Immunoresearch, Cat# 111-165-144) or alexa fluor 488 for 2 h at room temperature