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Hippocampal neurons were then cultured a further 3-7 days, at which time they were either transfected or treated with various pharmacological reagents as described in (Wayman et al., 2008)

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Here is a simple side-by-side of how long each method usually takes for common changes

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But with growth comes concerns

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Massage: Gently massage in upward circular motions until fully absorbed

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2-3 days interstate shipping

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