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The MSIA tips are then rinsed with a series of buffer and water rinses to remove salts and detergents ( Figure 1 , step 3), after which captured IGF-1/LR3 are eluted from the MSIA tips by iteratively pipetting an elution solvent of 33% acetonitrile in 0.4% trifluoroacetic acid, TFA (step 4)

Tanaka T, Yabusako T, Yamashita T et al

77 Increased excitatory neurotransmission, 78 enhanced non-auditory projections, 79 and changes in GABAergic transmission contribute to the excitatoryinhibitory imbalance