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8-12ml/g In the peptide cutting reagent, the concentration of the resin is 8-12ml/g C 18 C 8 According to the present invention, the method for purifying the crude product through C18 or C8 chromatographic column comprises the steps: A0.199.9B0.199.91-150ml/min215285nm There are two mobile phases: A: 0.1% trifluoroacetic acid plus 99.9% water

Donnelly J, Popovich PG